- Hot search：
ISO 19045-2015 pdf free download
ISO 19045-2015 pdf free download.Ophthalmic optics Contact lens care products – Method for evaluating
Acanthamoeba encystment by contact lens care products
Optique ophtalmique — Produits d’entretien de lentilles de contact — Méthode d’évaluation de l’enkystement de Acanthamoeba au contact des produits d’entretien des lentilles de contact.
18.104.22.168 Mix contents of wells by gently pipetting up and down three times with a 1 000 tl pipettor set to deliver 500 tl or a 3 ml disposable pipette.
22.214.171.124 Immediately perform cell chamber counts using the hemocytometer on each of the three To cncystment negative control wells and record the individual and averaged total cell count/mi in TabIel.
126.96.36.199 Measure the background cyst count in the inoculum by adding 50 uI of the Sarkosyl-Calcofluor White solution (see Annex C) to each of the three To encystment negative control wells pipetting vigorously to mix and leaving at room temperature for S mm.
NOTE It has been observed that 5 mEn is critical for optimal assessment. Longer exposure times can cause the cysts to lyse and become difficult to recognize.
188.8.131.52 Again, mix vigorously by pipetting up and down using a 1 000 iii pipettor set to deliver 500 il or a 3 ml disposable pipette.
184.108.40.206 Immediately perform cell chamber counts using the hemocytometer on the Sarkosyl-Calcofluor White treated cells (under UV fluorescence with appropriate filter for Calcofluor white detection) to determine the background cyst level. Switch between UV fluorescence and white light to confirm observation of cysts. Count the number of refractile and fluorescent cells/mi and record in Tablel. These represent immature and mature cyst forms and give the background levels for the experiment (see Eigiue2 for representative images of encysted trophozoites).
220.127.116.11 The test is invalid if the background cyst count in the challenge inoculum is greater than 5,0 %. If greater than 5,0 %, prepare fresh inoculum and repeat the procedure.
4.8.3 Test samples
18.104.22.168 Mix all solutions vigorously immediately before dispensing. Dispense each test solution directly from the product bottles into three wells In the microtitre plates as in
22.214.171.124 Add 30 ul of 1,0 x 107/ml trophozoites in 1/4 strength Ringer’s solution to all wells. This is designed to yield a final concentration of 1 x 105 trophozoites/mI in the test wells. If the stock trophozoite concentration is found to be >1,0 x 107/ml. then add an appropriate volume to the wells to yield the required inoculum concentration of 1 x 105 trophozoites/mI (e.g. 20 uI if the stock suspension was counted as 1,5 107/ml).
126.96.36.199 Mix contents of wells by gently pipetting up and down three times with a 1 000 ul pipettor set to deliver 500 p1 or a 3 ml disposable pipette.ISO 19045-2015 pdf free download.
All resources on this website are from the Internet. It is only for personal study and research. No one is allowed to use it for any commercial purpose. If you need, please buy the genuine standard. If there is any infringement of your copyright, please contact the webmaster in time, and I will correct it immediately.
- Maybe you like
- Latest Updates
- ISO 287-2009 pdf free download
- ISO 527-5-2009 pdf free download
- ISO 659-2009 pdf free download
- ISO 660-2009 pdf free download
- ISO 712-2009 pdf free download
- ISO 725-2009 pdf free download
- ISO 730-2009 pdf free download
- ISO 927-2009 pdf free download
- ISO 2041-2009 pdf free download
- ISO 3040-2009 pdf free download
- ISO 3506-2-2009 pdf free download
- ISO 3506-3-2009 pdf free download